Composite

Part:BBa_K2378005:Design

Designed by: Muhammad Farhan Maulana, Marvin Nathanael Iman, Zulhaerana Bahar   Group: iGEM17_ITB_Indonesia   (2017-10-27)


Constitutive PETase Coding Device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 70
    Illegal NotI site found at 979
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 724
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence is optimized for E. coli BL21 by synonymous mutation. The original sequence is based on http://www.uniprot.org/uniprot/A0A0K8P6T7


Source

Ideonella sakaiensis

References